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pcag flpe gfp  (Addgene inc)


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    Structured Review

    Addgene inc pcag flpe gfp
    Pcag Flpe Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+pcag+flpe+gfp/pCAG-Flpe%3AGFP+(Plasmid+%2313788)/pmc12728157-422-21-22
    Average 93 stars, based on 16 article reviews
    pcag flpe gfp - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Detection of single enzymatic events in rare or single cells using microfluidics.
    Article Snippet: .. Plasmid pCAG-Flpe:GFP for expression of Flpe C-terminally tagged with green fluorescent protein (GFP) in human cells was from Addgene. .. Transient transfection of pCAG-Flpe:GFP into HEK293 cells was performed using Lipofectamine2000 (Invitrogen) and 8 μg of plasmid DNA and was carried out in Gibco'S reduced serum medium (OPTI-MEM) according to the manufacturer's instructions.

    Article Title: Detection of Single Enzymatic Events in Rare- or Single Cells Using Microfluidics
    Article Snippet: The cell densities were adjusted to 0.5-5 million cells/mL and used for enzyme activity detection in the microfluidic system. .. Plasmid pCAG-Flpe:GFP for expression of Flpe C-terminally tagged with green fluorescent protein (GFP) in human cells was from Addgene. .. Transient transfection of pCAG-Flpe:GFP into HEK293 cells was performed using Lipofectamine2000 (Invitrogen) and 8 μg plasmid DNA and was carried out in GIBCO’s Reduced Serum Medium (OPTI-MEM) according to the manufacturer’s instructions.

    Article Title: γ-Secretase modulator resistance of an aggressive Alzheimer-causing presenilin mutant can be overcome in the heterozygous patient state by a set of advanced compounds.
    Article Snippet: Next, the Ngn2 gene was inserted into the vector pZ M2rtTA_CAGG TetON-Sox10 with GFP [41] (Addgene, Cat. No. 115241, kindly provided by Prof. Verfaille (Stem Cell Institute Leuven, KU Leuven, Leuven, Belgium)) by replacing Sox10 using EcoRV/AflII, so that it can be expressed under a DOX-inducible promoter. .. The purified Ngn2-containing plasmid was transfected into the human master iPSC line together with a flippase-encoding plasmid (pCAG-Flpe-GFP, a gift from Connie Cepko, Addgene, Cat. No. 13788). ..

    Article Title: System for identification of microorganism and detection of infectious disease
    Article Snippet: The cell densities were adjusted to 0.5-5 million cells/mL and used for enzyme activity detection in the microfluidic system. .. Plasmid pCAG-Flpe:GFP for expression of Flpe C-terminally tagged with green fluorescent protein (GFP) in human cells was from Addgene. .. Transient transfection of pCAG-Flpe:GFP into HEK293 cells was performed using Lipofectamine2000 (Invitrogen) and 8 μg plasmid DNA and was carried out in GIBCO's Reduced Serum Medium (OPTI-MEM) according to the manufacturer's instructions.

    Expressing:

    Article Title: Detection of single enzymatic events in rare or single cells using microfluidics.
    Article Snippet: .. Plasmid pCAG-Flpe:GFP for expression of Flpe C-terminally tagged with green fluorescent protein (GFP) in human cells was from Addgene. .. Transient transfection of pCAG-Flpe:GFP into HEK293 cells was performed using Lipofectamine2000 (Invitrogen) and 8 μg of plasmid DNA and was carried out in Gibco'S reduced serum medium (OPTI-MEM) according to the manufacturer's instructions.

    Article Title: Detection of Single Enzymatic Events in Rare- or Single Cells Using Microfluidics
    Article Snippet: The cell densities were adjusted to 0.5-5 million cells/mL and used for enzyme activity detection in the microfluidic system. .. Plasmid pCAG-Flpe:GFP for expression of Flpe C-terminally tagged with green fluorescent protein (GFP) in human cells was from Addgene. .. Transient transfection of pCAG-Flpe:GFP into HEK293 cells was performed using Lipofectamine2000 (Invitrogen) and 8 μg plasmid DNA and was carried out in GIBCO’s Reduced Serum Medium (OPTI-MEM) according to the manufacturer’s instructions.

    Article Title: System for identification of microorganism and detection of infectious disease
    Article Snippet: The cell densities were adjusted to 0.5-5 million cells/mL and used for enzyme activity detection in the microfluidic system. .. Plasmid pCAG-Flpe:GFP for expression of Flpe C-terminally tagged with green fluorescent protein (GFP) in human cells was from Addgene. .. Transient transfection of pCAG-Flpe:GFP into HEK293 cells was performed using Lipofectamine2000 (Invitrogen) and 8 μg plasmid DNA and was carried out in GIBCO's Reduced Serum Medium (OPTI-MEM) according to the manufacturer's instructions.

    Purification:

    Article Title: γ-Secretase modulator resistance of an aggressive Alzheimer-causing presenilin mutant can be overcome in the heterozygous patient state by a set of advanced compounds.
    Article Snippet: Next, the Ngn2 gene was inserted into the vector pZ M2rtTA_CAGG TetON-Sox10 with GFP [41] (Addgene, Cat. No. 115241, kindly provided by Prof. Verfaille (Stem Cell Institute Leuven, KU Leuven, Leuven, Belgium)) by replacing Sox10 using EcoRV/AflII, so that it can be expressed under a DOX-inducible promoter. .. The purified Ngn2-containing plasmid was transfected into the human master iPSC line together with a flippase-encoding plasmid (pCAG-Flpe-GFP, a gift from Connie Cepko, Addgene, Cat. No. 13788). ..

    Transfection:

    Article Title: γ-Secretase modulator resistance of an aggressive Alzheimer-causing presenilin mutant can be overcome in the heterozygous patient state by a set of advanced compounds.
    Article Snippet: Next, the Ngn2 gene was inserted into the vector pZ M2rtTA_CAGG TetON-Sox10 with GFP [41] (Addgene, Cat. No. 115241, kindly provided by Prof. Verfaille (Stem Cell Institute Leuven, KU Leuven, Leuven, Belgium)) by replacing Sox10 using EcoRV/AflII, so that it can be expressed under a DOX-inducible promoter. .. The purified Ngn2-containing plasmid was transfected into the human master iPSC line together with a flippase-encoding plasmid (pCAG-Flpe-GFP, a gift from Connie Cepko, Addgene, Cat. No. 13788). ..



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